Aplied Physics Researches Division (APRD)

Radiation Biophysics Researches

Posted on Dec 15, 2015

Radiation Biophysics Researches

 

Investigation of microbiological destruction of xenobiotics/pesticides.

  • Scanning of the cultures of microorganisms is performed in the collection of cultures stored at the Laboratory of radiation biophysics to find out the cultures with tolerance to the presence of high concentration of pesticides. A number of strains capable to destroy herbicides metazine and propazine are revealed. Only strain is found out capable to grow effectively in the medium containing insecticide actara. No culture capable to grow effectively in the presence of insecticide confidor was revealed. In all cases, pesticides served as the only source of nutrition (as the source of carbon and nitrogen).
  • With the purpose of collection, an extensive site survey enlargement was organized with the active support of the Ministry of ecology, and several samples of soil from the territory of the Nubarashen toxic chemicals burial were taken. From the samples of soil taken from various sites of the burial, few tens of strains were isolated, some of which had revealed capability to grow in the medium containing confidor, and some more – rather intensively growth on actara.
  • The cultures grown on actara have shown the ability to form «halo» – a nimbus around the colony, the phenomenon testifying intensive catabolic processes in the cells of microorganisms. The character of the halo formation specifies that the enzymes involved in the process are most likely of an inducible nature.
  • The preliminary results on shake-flask fermentations have shown a weak (within 10 %) ability of one of the selected cultures to utilize the actara.
  • During the experiments on shake-flask fermentations, mutagenic influence of actara on the strains grown in its presence was revealed.

Obtaining of mutant strains with higher purposeful destructive activity using ionizing and non-ionizing radiations.

¡Investigation of biological influence of electrons with the energy 7.5 MeV on microorganisms

Some differences on survival curves of the E. Coli strains of different genotypes were revealed depending on the electron linear energy transfer in comparison with with other type of irradiations.

Investigation of the impact of Armenian NPP on microbiota of soils around the NPP.

This cycle of investigations was performed during the reporting period in the frame of ISTC project A-773 (“Net Effect of the Armenian Nuclear Power Plant on Environment and Population in the Background of Global Radioactive Fallouts”). It will be very useful to repeat this cycle of research to confirm this result and estimate reasonableness of the use of microorganizms as bioindicators of radiation impact.

Investigation of radioprotection properties of proline rich polypeptides of brain

  • It has been experimentally shown that 1 mkg PRP increase the radioprotectiviti of nonlinear mice at the LD50/30 dose of X-ray radiation
  • The 1 mkg PRP, which was given to mice by intramuscular injection 24 hours before radiation, provides 100% survivability at the X-ray irradiation of LD 50/30 dose, and simultaneously it was registered 50% lethality in 30 day period in the control group.
  • In the experimental group of animals (injected PRP) there was no registered lethality in 60 day period after 30 day post radiation period, which means that PRP removes influence of X-ray radiation in long postradiation period.
  • For experimental determination of free radical interactions in tissues under the influence of X-ray radiation was projected and designed luminometer, which is equipped with a Hamamatsu H9319-02 (Hamamatsu Japan) multi-alkali photomultiplier and the National Instruments data acquisition card NI DAQ .